What is the fail_reads.bam file?
On the Revio platform, for each hifi_reads.bam there is one fail_reads.bam file. This file contains one representative read per ZMW. The ff tag encodes why each read ended up in this file:
| Flag | Description | 
|---|---|
0x1 |  CCS reads with predicted accuracy below QV 20. | 
0x2 |  Control CCS reads. | 
0x4 |  Single-stranded CCS reads. | 
0x8 |  The median full-length subread from molecules that do not produce a CCS read but have at least one full pass. | 
0x10 |  CCS reads which are a concatenation of the adapter, with possible short non-adapter sequence in between. | 
0x20 |  CCS reads with miscalled adapter which is enclosed by a sequence and its reverse complement, either spanning to the end. | 
0x40 |  CCS reads that have one or more adapters close to either end. |