ccs combines multiple subreads of the same SMRTbell molecule using a statistical model to produce one highly accurate consensus sequence, also called a HiFi read, along with base quality values. This tool powers the Circular Consensus Sequencing workflow in SMRT Link for Sequel and Sequel II platform. On Sequel IIe and Revio platforms, HiFi generation is perfomed on the instrument.
A New Paradigm in Sequencing with HiFi Reads
Advanced scientific discoveries require sequencing data that is both accurate and complete. PacBio sequencing technology has evolved to a different type of long read, known as highly accurate long reads, or HiFi reads. PacBio is the only sequencing technology to offer HiFi reads that provide accuracy of 99.9%, on par with short reads and Sanger sequencing. With HiFi reads you no longer have to compromise long read lengths for high accuracy sequencing to address your toughest biological questions.
Availability
ccs version 6.4.0 for SQII can be installed via the bioconda package pbccs.
Please refer to our official pbbioconda page for information on Installation, Support, License, Copyright, and Disclaimer.
Latest Version
Version 8.0.1: Full changelog here
What’s new!
- DeepConsensus is integrated into ccs for the Revio platform
- Output BAM file compression
- Add fail flag
ff
Schematic Workflow
Execution
Input: Subreads from a single movie in PacBio BAM format (.subreads.bam).
Output: Consensus reads in a format inferred from the file extension: unaligned BAM (.bam); bgzipped FASTQ (.fastq.gz); or SMRT Link XML (.consensusreadset.xml) which also generates a corresponding unaligned BAM file.
Run on a full movie:
ccs movie.subreads.bam movie.ccs.bam
Parallelize by using --chunk. See how-to chunk.
Feel free to make ccs slightly faster via environment variables.